Ubiquitin Serum

Ubiquitin Serum is used for the detection, quantification, and study of ubiquitin and ubiquitin-related pathways in biological samples. It is primarily utilized in research to understand protein degradation, signal transduction, and cellular regulation.

Key Components

• Ubiquitin: Contains ubiquitin proteins, which are small peptides involved in tagging proteins for degradation or modifying their functions through ubiquitination.
• Buffer System: Includes a buffer to maintain pH and ionic strength suitable for preserving ubiquitin's structure and function.
• Stabilizers: Agents to prevent degradation of ubiquitin and maintain the serum’s efficacy over time.

Technical Specifications

• Ubiquitin Concentration: Typically provided in µg/mL or mg/mL, optimized for various experimental conditions.
• Purity: High-purity ubiquitin is used, often >95%, to ensure reliable and specific interactions in assays.
• Storage Conditions: Stored at -20°C or -80°C for long-term stability. Avoid repeated freeze-thaw cycles to maintain integrity.

Applications

• Western Blotting: Used to detect ubiquitin-conjugated proteins and analyze ubiquitination patterns.
• ELISA: For quantitative measurement of ubiquitin levels in samples.
• Immunoprecipitation: To isolate ubiquitin-conjugated proteins and study their interactions.

Usage Instructions

• Dilution: Dilution factors should be optimized based on the specific assay requirements. Refer to the manufacturer’s guidelines for recommended dilutions.
• Compatibility: Ensure that the serum is compatible with other reagents and samples in the assay system to avoid interference.

Quality Control

• Validation: Each batch is tested for purity, activity, and specificity to ensure consistent performance.
• Performance Metrics: Regular assessment is performed to maintain high standards in reproducibility and accuracy.

Safety Information

• Handling: Handle with care, following standard laboratory safety protocols. Use appropriate personal protective equipment (PPE) and avoid direct contact with skin or eyes.
• Disposal: Dispose of according to local hazardous waste regulations.